Storage of human milk for further use in infant feeding, now is more frequently, as a result of the
social- economic activities of breastfeeding mothers. Expressed breast milk is usually stored frozen for
consumption at a later time. Currently, human milk is stored in containers with headspace, where the removal of
oxygen should minimize the oxidative effects in compounds of stored breast milk during shelf life, and improve
nutritional quality.
The purpose of this study was to analyze the quantity of some vitamins and compare any variation in vitamin C & some selected vitamins B (B1, B2, B3) in milk frozen in two different containers; one with air in the
headspace versus vacuum sealed storage containers. Vitamin C content were analyzed using high-performance
liquid chromatography (HPLC) Vitamin B1, B2, and B3 were also analyzed using HPLC. Fresh, mature, human
milk donated by 4 women with healthy infants was used for analysis. Infant formula as powder and rehydrates
liquid was also analyzed. According the results of analyses, the values of infant formula nutrient are generally
higher than the human milk values.
Author Keywords:- high-performance liquid chromatography, human milk, infant formula, Vitamin, B1, B2, B3, C.
e-ISSN: 2319-183X, p-ISSN: 2319-1821 Source Type: Journal
Original Language: English
Document Type: Article
Number of pages: 03
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